EHT   - the beat 
of advanced cardiac science

Aboard the effortless journey from lab to life

From the intricacies of disease modeling to the frontiers of gene therapy, EHT provides a robust platform for breakthroughs in personalized medicine and beyond.
Learn more about EHT
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We aim to make the human body measurable. One organ at a time.

Starting with the heart.

Smiling woman with white blouse, professional headshot.
Alexandra Rhoden, PhD
Head Research & Technology

Engineered Heart Tissues (EHTs) are three-dimensional, fibrin-based cardiac constructs derived from human induced pluripotent stem cells (hiPSCs). Our innovative technology combines hiPSCs with 3D tissue engineering to simulate human heart tissues, enabling precise cardiac safety and efficacy testing, disease modeling and precision medicine including gene therapy applications.

EHTs not only facilitate the rapid translation of research findings into preclinics but also provide a powerful testbed for personalized medicine approaches.

1.1 

Human iPSCs

Human iPSCs serve as the fundamental cell source for human tissues. Derived from healthy individuals or patients with specific diseases, these versatile cells can be reprogrammed from various sources such as blood, skin, or urine.

1.2 

Beating Cardiomyocytes

Using specialized protocols, hiPSCs are differentiated into cardiomyocytes with high purity. This transformation is critical because these beating heart cells are the active component of the engineered heart tissue (EHT).

1.3 

Cast EHTs

In this step, cardiomyocytes are embedded in a liquid hydrogel and positioned around silicone posts. The hydrogel polymerization fosters tissue formation between these posts¹, creating the structural backbone of the EHTs.

1.4 

Maturation of hiPSC-CM

Here, cardiomyocytes elongate along force lines. The stretch applied by silicone posts mimics the native cardiac environment and enhances the maturation of these cells in 3D. This process can be accelerated with electrical stimulation and special media formulations.

1.5 

Unique Model (Patent)

Our patented technology, a first in the field of cardiac tissue engineering, represents a unique model. US Patent No. 7,618,452 and EU Patent EP1250416B1 encapsulate our pioneering approach to the development of EHTs.

Wouldn’t that be nice: A system that helps you discover breakthroughs with ease. That’s why we developed the DiNAQUBE™!

A fully automated EHT contraction measurement system: Its cameras capture non-invasively. It recognizes tissue with machine learning. And does it all fast: From start to result in just a minute and a half!

Now you can focus on interpretation instead of data management.

Sterile video-optical measurements

Conduct sterile, non-invasive video-optical 
measurements for in-depth, long-term studies
No more compromising study length due 
to invasive techniques.

24 EHTs in 90 seconds

Analyze up to 24 Engineered Heart Tissues simultaneously (the fastest solution on the market).
No more waiting thanks to accelerated throughput.

Automated tissue recognition

Use machine learning-based tissue recognition for consistent, reliable results every time.
No more manual, error-prone tissue identification.

Controlled environment for precise EHT analysis

Achieve precise EHT analysis in a specialized, controlled environment incubator.
No more struggling with environmental variables and biased results.

Real-time contraction analysis

Benefit from real-time analysis of muscle contractions 
with automatic figure recognition.
No more time-consuming manual contraction tracking.

Integrated Rate Control Analysis

Perform frequency-driven analysis with built-in stimulation signals for detailed contractile kinetics.
No more manual setting and monitoring of pacing signals for contraction analysis.

Advanced Force and Kinetic Calculations

Automatically calculate force based on EHT shortening, geometry, and rack elasticity.
No more complex manual calculations for force and kinetic data.

The power to quickly dive deep into your data comes with our 
web-based software.

It allows unprecedented comparison of multiple measurements across different plates, all in a single view. Visualized results give you clarity. And machine learning capabilities help you categorize EHTs.

All done intuitively with a few clicks. Your research should be this easy.

Multidimensional data analysis

Comprehensive query functions allow you to explore and navigate multiple dimensions of data. View and compare multiple measurements simultaneously in one view.

Endless comparison possibilities

Review a vast number of parameters and relate dimensions (such as plate name, well position or EHT age) to measurements (such as force or frequency).

Big data, bigger insights

Seamlessly integrate disparate data sets for comprehensive analysis at the click of a button.

Various exploration functions

You can filter, sort, group, and slice data based on different criteria. Or apply a function to specific values.

Improve accuracy with ML

Automated quality control and machine learning help simplify classification and ensure data integrity, user-independent.

Multiple visualization options

Choose from a variety of presentation formats, such as table views, scatter & line plots, images, and video directly from the database for in-depth data analysis.

Snapshot function

You can take snapshots of data at specific points in time, which is critical for tracing and reproducing research results.

All benefits at a glance

Meet our proprietary 3D automated measurement system and DiNALYTIQS™
Advanced technology for human heart tissue engineering
read more

Human iPSC-derived cardiomyocytes more closely resemble human cardiac physiology than animal models.

Contraction against an elastic resistance, qualitatively comparable to the load imposed on real human heart.

3D cardiac tissues reflect all compensatory mechanisms of the human heart.

Non-invasive measurements with balanced conditions.

3R compliant, human based heart model


Provides a humane alternative to animal testing in preclinical toxicology assessments

Awarded for adherence to the 3R principles of “replace, reduce, refine” in animal research*

Aligns with ethical guidelines and industry best practices, ensuring a clear conscience in research methodology

Scalable, automated solutions for streamlined research

Rapid screening with the ability to analyze 24 cardiac tissues simultaneously in 90 seconds

Increased efficiency through automated feeding and screening street

Flexibility to integrate customized secondary assays as per research needs

Access to both in-house and commercially available cardiomyocytes, ensuring comprehensive data and analysis

Efficient and intelligent data handling

Autonomous figure recognition and parameter calculation, independent of the user

Comprehensive statistics, plots and summary reports at your fingertips

Intelligent classification of contractility and tissue samples through AI

Seamless integration with big data resources for enhanced information retrieval

Consistent and reliable long-term sterile testing

Enables both short- and long-term exposure of new drug candidates

Provides a highly robust, reproducible and automated platform for controlled environment measurements with electrical stimulation options

Authentic human cardiac model for superior toxicology

Captures relevant parameters for toxicology screening, surpassing current non-human in vitro models, animal models and human heart muscle trabeculae (dying preparations)

Offers integrated functional (contractility) analyses, providing a high-content assay with the potential for additional readouts

Human iPSC-derived cardiomyocytes more closely resemble human cardiac physiology than animal models

Contraction against an elastic resistance, qualitatively comparable to the load imposed on real human heart

3D cardiac tissues reflect all compensatory mechanisms of the human heart

Non-invasive measurements with balanced conditions

Provides a humane alternative to animal testing in preclinical toxicology assessments

Awarded for adherence to the 3R principles of “replace, reduce, refine” in animal research

Aligns with ethical guidelines and industry best practices, ensuring a clear conscience in research methodology

Rapid screening with the ability to analyze 24 cardiac tissues simultaneously in 90 seconds

Increased efficiency through automated feeding and screening street

Flexibility to integrate customized secondary assays as per research needs

Access to both in-house and commercially available cardiomyocytes, ensuring comprehensive data and analysis

Autonomous figure recognition and parameter calculation, independent of the user

Comprehensive statistics, plots, and summary reports at your fingertips

Intelligent classification of contractility and tissue samples through machine learning

Seamless integration with big data resources for enhanced information retrieval

Enables both short- and long-term exposure of new drug candidates

Provides a highly robust, reproducible and automated platform for controlled environment measurements with electrical stimulation options

Captures relevant parameters for toxicology screening, surpassing current non-human in vitro models, animal models, and human heart muscle trabeculae (dying preparations).

Offers integrated functional (contractility) analyses, providing a high-content assay with the potential for additional readouts

Ready to research with EHT?

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